The story of replication, transcription and translation.

	Replication	Transcription	Translation
Initiation	- helicase - single stranded binding protein keep template strands apart - gyrase releases tension from the unwinding DNA - primase (A to U, C to G)	- All in the initiation complex found in the promoter region - transcription factors (proteins) - TATA box on DNA - RNA II (protein and template)	- tRNA, mRNA, the first amino acid and two ribosomal subunits are brought together - MET, the start codon - read 5’ AUG 3’
Elongation	- DNA polymerase III elongates from 5’ to 3’ and reads 3’ to 5’	- Known as the Transcription Unit - RNA polymerase II reads DNA 3’ to 5’, ultimately creating RNA 5’ to 3’ that is similar to the coding strand	- read mRNA from 5’ to 3’ - codon recognition occurs at the A-site with the bonding of mRNA with the anticodon - peptide bond formation with amino acids from A site and polypeptide at P site - ribosome move tRNA with polypeptide from A to P site for translocation.
Termination	- DNA polymerase I proofreads and replaces RNA primers with DNA nucleotides - ligase ‘glues’ the gaps	- AAUAAA sequence (RNA) stops the production of RNA - pre mRNA is created	- stops upon reaching a stop codon: UAA, UAG, UGA